There are now fresh calls for the Coalition to preference One Nation last at the federal election following revelations that Pauline Hanson appeared to question the 1996 Port Arthur massacre, which left 35 people dead in Tasmania.

Her comments have prompted Liberal backbencher John Alexander to expect his party would have to preference One Nation last at the next election.

The footage of Senator Hanson is the second instalment in an Al Jazeera documentary showing her having a discussion about Port Arthur with her chief of staff James Ashby and undercover journalist Rodger Muller.

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