Federal Court action will be launched against Australian travel giant Flight Centre today, alleging the systemic underpayment of employees — a claim the company rejects.

The claim is being brought on behalf of five current and former employees by Maurice Blackburn Lawyers and the union, Together Queensland.

Maurice Blackburn's Employment Law Principal Giri Sivaraman said the company failed to pay minimum wages, penalty and overtime rates, annual leave and leave loading at the correct rate and did not provide the correct rest and meal breaks.

Mr Sivaraman says by their calculations, in total, the employees have missed out on about $250,000, a quite significant sum he says.